Buckyballs: study finds toxicity, and a cure
reduce the toxicity of water-soluble buckyballs by a factor of more
than ten million.
The research - which could open the door for the use of this much-researched form of carbon as a drug delivery vehicle - will appear in an upcoming issue of the journal Nano Letters, published by the American Chemical Society. The research was published online by the journal on 11 September.
Buckyballs, whose chemical notation is C60, are hollow, soccerball-shaped molecules containing 60 carbon atoms, also known as buckminsterfullerene. Their diameter is just one nanometer, and their discovery at Rice in 1985 is regarded as an early milestone in the field of nanotechnology.
While buckyballs show great promise in applications as diverse as pharmaceuticals, fuel cells, batteries, and coatings, some scientists and activists have raised concerns about their potential toxicity to humans and animals.
Rice University's study is the first cytotoxicity experiment on human cells exposed to buckyballs.
The study found that even minor alterations to the surface of the buckyballs can dramatically affect how toxic they are to individual cells, and the researchers identified specific alterations that render them much less toxic.
"There are many cases where toxicity is desirable," said Vicki Colvin, professor of chemistry and chemical engineering at Rice, and the principal investigator for the research. "For example, we might want particles that kill cancer cells or harmful bacteria. In other cases - like applications where particles may make their way into the environment - toxicity is undesirable."
In the study, the researchers exposed two types of human cells to various solutions containing different concentrations of buckyballs. Four types of solutions were tested. One contained tiny clusters of smooth-surfaced buckyballs. In the other three, researcher s modified the buckyballs by attaching other molecules to their sides. Researchers measured how many cells died within 48 hours of exposure to each solution, and they repeated the tests until they found the exposure level for each that resulted in a 50 per cent mortality rate.
In general, the greater the degree of surface modification, the lower the toxicity. For example, the undecorated buckyballs showed the highest toxicity -- about 20 parts per billion-- while the least toxic proved to be buckyballs decorated with the largest number of hydroxyl side-groups. To achieve the equivalent level of toxicity as that of bare buckyballs, the researchers had to increase the concentration of these modified buckyballs by 10 million times to more than 5 million parts per billion.
"We're encouraged to see that controlling the surface properties of buckyballs allows us to dial the level of toxicity up or down, because making those kinds of modifications is something that chemists do every day in university research labs and in industry," Colvin said.
Moreover, she said the technique could also prove useful in tuning the toxicity of other nanoparticles.
The researchers postulate that cell death in the tests occurred via physical disruption of the cell membrane by oxygen radical species generated by the buckyballs.
Colvin and her colleagues emphasise that the study only fills in part of the puzzle regarding fullerene toxicity. For example, because cytotoxic studies look only at cells in culture, they say little about what happens inside the body, where cellular repair mechanisms, whole-organ and whole-body processes come into play.
"Cytotoxicity should not be confused with a full-fledged toxicological risk assessment," said Kevin Ausman, a co-author of the paper. He added that risk assessments take into account exposure rates, uptake mechanisms, transport within the body and other issues.
"But based on our results we think buckyballs should be studied in more detail, and we're already working to arrange additional studies," said Ausman.
