Nikon launches spectral imaging detector
detector that captures images faster with a single scan over the
full spectral range. The quality of images obtained from multiple
fluorescence probes within a sample will have numerous
applications, particularly in live cell biology.
The power of the new C1si is fully realised in modern research where there is a need to use a plethora of fluorochrome markers. It can capture data over a wide spectral range encompassing fluorescence proteins such as CFP, GFP, YFP, DsRed in one hit, and conversely separate the signals of close spectral emitters GFP & FITC.
The sensitivity, fast image capture and signal to noise characteristics of the C1si makes it suitable for the high data acquisition rates necessary for live cell spectral imaging. The researcher can extend the life of cells under observation by reducing phototoxicity and photobleaching, whilst easily capturing dynamic and kinetic changes to the cells.
The system incorporates confocal technology that acquires high-resolution data, over a spectral range of 400-750nm, in a single scan providing the researcher with continuous spectral imaging content.
Traditionally, fluorescence solutions have difficulty in cleanly separating signals from fluorescing probes and sample auto-fluorescence, due to overlap of their fluorescence emission spectra. Taking a step up to a confocal solution, usually resolves this, but this is limited by collecting data at specific wavelengths only.
This can be especially troublesome in FRET microscopy where precise localisation of the source of the signal is required. In addition to this the time taken to capture such information can potentially hinder some research into dynamic cell activity.
The Eclipse C1si Spectral Laser Confocal system can acquire image data over a 320nm wide spectral range, in 10nm wavelength resolutions or over smaller ranges at 5nm or 2.5nm resolutions, in less than 1s at 512 x 256pxl. This is possible by utilising a 32 PMT channel detector and not placing dependence on the confocal pinhole size.
Furthermore, the Digital Eclipse C1si employs a mechanism to prevent illuminating wavelengths from blinding the detector. The emission path can be easily switched between brightfield, differential interference contrast, standard three-channel fluorescence detection and the high-resolution spectral detector, allowing a single instrument to be used for the widest possible range of applications.
The optical design and signal processing of the Digital Eclipse C1si allows for a brighter and sharper image. The combination of the newly developed Diffraction Efficiency Enhancement System (DEES), fluorescence transmission technology, optimises the signal reaching the photomultiplier tubes, thus making the image brighter and sharper.
The Dual Integration Signal Processing (DISP) technology also eliminates digitisation dead time. The combination of DISP & DEES technology ensures spectral imaging with the best signal to noise ratio possible.
The Eclipse C1si Spectral Laser Confocal system is now available to order from Nikon's website.
