The Eclipse Plus columns are an expansion to Agilent's Zorbax column family and are made using an improved silica-manufacturing and novel bonding process under stringent QA/QC and lot-to-lot reproducibility standards.
The entire process is designed to consistently generate symmetrical peaks and superior resolution, even for the most difficult separations.
The new >Agilent Zorbax Eclipse Plus LC columns deliver improved peak shape for the most difficult basic compounds along with highly reproducible performance, resulting from Agilent's rigorous product QA/QC testing.
In addition, the Eclipse Plus is available in 1.8, 3.5 and 5 um particle sizes for all analytical, high-resolution and fast LC analyses.
"Zorbax Eclipse columns offer a best-in-class solution that lets users optimise all their LC analyses within a single family of columns," said Helen Stimson, Agilent vice president and general manager, Global Columns and Supplies Solutions, Life Sciences and Chemical Analysis.
HPLC, is a form of column chromatography used frequently in biochemistry and analytical chemistry. HPLC is used to separate components of a mixture based on a variety of chemical interactions, usually of a non-covalent nature, between the analyte and the chromatography column.
The end result is that chemical compounds which start mixed in a single sample injected into the instrument are eluted (come out of the instrument) at different times.
These peaks, each representing a different chemical species, may be collected as seperate purified compounds or more often simply detected to determine their presence or quantity.
Zorbax Eclipse LC columns are well-suited for method development of all samples. They are available in a wide selection of dimensions, particle sizes and bonded phases that work across a wide pH range and separation conditions.
Methods can be scaled from analytical to nano scale or preparatory scale, all within the same column family. Conventional HPLC methods can be converted to fast or ultra-fast high-throughput chromatography without changing selectivity.
The new offerings include a line of Eclipse Rapid Resolution High Throughput (RRHT) 1.8 um LC columns designed to optimise the performance of Agilent's new 1200 Series Rapid Resolution LC system, its high-resolution separation platform.