Single-pass tangential flow filtration (SPTFF) is a method of ultrafiltration for the purposes of either volume reduction or protein concentration. This versatile method has been successfully implemented between unit operations throughout the downstream production process of biopharmaceuticals. Its value lies in the simplicity of operation as well as its small footprint and equipment requirement. This document describes the principles of SPTFF and how to determine operating conditions to achieve the target concentration factor with single-use Pellicon® Capsules.
