Eppendorf doubles up for gene expression

German instrument, consumable and reagent company Eppendorf has introduced a new range of low-density DNA microarrays for differential gene expression analyses.

The DualChip line differs from traditional microarrays in each slide incorporates two identical DNA microarrays, each surrounded by a closed hybridisation chamber.

The microarrays are designed for use with Eppendorf​'s Exchangeable Thermoblock For Slides, a climatic chamber introduced earlier this month specifically for use with the DualChip range. The chamber provides consistent conditions with respect to temperature and air humidity when used in conjunction with Eppendorf's Thermomixer comfort for mixing or ThermoStat plus for temperature control.

The new Thermoblock product incorporates a liquid reservoir to stop the microarray slides drying out (which can cause increases in background signals). When used in combination with the Thermomixer to improve the probability of contact between the target molecule and the array, the signal intensity of microarray experiments increases by a factor of 2.5 and 4, depending on the level of gene expression.

The DualChip arrays are designed with a series of gene-specific probes , including in silico​ and in situ​-optimised products for toxicology, stress and ageing, G-protein-couple receptors and general screening.