Argonaut senior VP endorses Isolute ppt+
ppt+ protein precipitation plates can overcome two major obstacles
holding back the purity of protein precipitation prior to LC-MS
analysis of drugs and metabolites in biological fluids.
The new technology eliminates the common problem of unwanted plasma proteins in biological fluid samples prior to analysis of drug candidates and metabolites.
In addition the technology uses a two-frit design at the bottom of each well preventing the wells from leaking or becoming clogged, increasing the plate yield and lowering the overall cost of sample preparation.
In an interview with DrugResearcher.com, Jeff Labadie, senior vice president of chemistry at Argonaut Technologies said that the design of Isolute ppt+ allowed the "solvent first" method for more complete precipitation. This resulted in cleaner samples across the whole plate and excellent recovery from all sample wells.
Labadie added: "We felt the robustness and ease of use of products in the market were lacking, including our current version of protein precipitation plates."
"The Isolute ppt+ allows for cleaner extracts without the need for extra vartex mixing steps. Filtration leads to 96 prepared samples for analysis."
Protein precipitation is a common sample preparation method used in bioanalytical analysis. There are two main approaches. One is to precipitate in tubes followed by centrifugation and separation of the sample from proteins by transferring the supernatant.
The other method is to precipitate in a special filterplate followed by filtration into a 96 well collection plate. The samples are then analysed by LC-MS.
Labadie added that the technology would be suitable for sample preparation of biological fluids for bioanalysis to support ADME, toxicology and pharmacokinetic studies in drug discovery. In addition, it can be used for sample preparation in pre-clinical experiments too.
The technology, which is aimed at bioanalytical chemists at pharma, biotechnology and contract research companies will be available worldwide with the fixed well plates priced from £45.00 (€68.00) and the versatile Isolute array plate priced from £48.00.
