Applied Bio system speeds PCR
polymerase chain reaction (PCR) experiments in 96-well plates that
can dramatically cut the time it takes to carry out the procedure.
PCR is a method for enzymatic amplification of a specific DNA sequence of interest. This technique is capable of amplifying a sequence 105 to 106-fold from tiny (nanogramme) amounts of DNA from a mixed sample, such as might be found in genomics studies.
Conventional methods require approximately two hours to carry out 30 amplification 'cycles', but the new 9800 Fast PCR System delivers PCR results in a standard 96-well plate in approximately 25 minutes, according to Applied Biosystems. The new product will be on exhibit at the upcoming American Society of Human Genetics Annual Meeting in Toronto, Canada, next week.
The 9800 Fast PCR System takes three existing products and puts them together: it consists of the 9800 Fast Thermal Cycler, the Applied Biosystems' GeneAmp Fast PCR Master Mix, and its 96-well Fast Thermal Cycling Plate. The system is expected to enable researchers to perform routine PCR procedures, such as cloning and preparing DNA for sequencing, faster, more easily, and more conveniently.
"In typical laboratory environments scientists often share their PCR instruments. Our new high-speed system can alleviate some of the burden of instrument sharing by reducing cycling time, thereby boosting productivity," said Peter Dansky, senior director and general manager of core PCR at Applied Biosystems.
"In addition, this system can help service and core laboratories increase their output and speed project timelines," he added.
More information on the new product can be found in an on-line 'webinar' that will take place on 9 November. To register, please visit http://info.appliedbiosystems.com/9800webinar.
